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64 Inhibition of HIV-1 Infection of Human Cell Lines by Expression of Ovine IFN-tau Gene Introduced with Spuma Virus Vector
Y. Murase*1, S. Omoto1, K. Otake2, Y. Yokota2, A. Ikemoto1, H. Okuyama1, Y. Yokomizo3, K. Imakawa4, and Y. Fujii1
1Nagoya City Univ., Japan; 2Natl. Inst. of Infectious Disease, Japan; 3Natl. Inst. of Animal Hlth., Japan; and 4Univ. of Tokyo, Japan
Spuma foamy viruses (FVs) have been isolated from a variety of animal species, including humans, felines, and bovines. The FV is apathogenic in vivo, but when fibroblastic cells are infected in vitro, FV is highly cytopathic. The FV genome contains 3 structural genes (gag, pol, and env) and 3 auxiliary genes (bel-1, -2, and -3). It has been reported that a foreign reporter gene is able to replace some or all of the bel-2 gene regions. Ovine interferon-tau (oIFN-tau) is produced by the trophectoderm in utero during the peri-implantation period. Recently, it has been reported that exogeneous supplement of a new anti-viral reagent, oIFN-tau, has inhibitory activity of human or feline immunodeficiency virus (HIV or FIV) replication in vitro; however, it has not yet been studied whether endogeneous or intracellular production of oIFN-tau is effective in the inhibition HIV replication. Therefore, to investigate the effect of oIFN-tau, we introduced oIFN-tau?gene into the foamyvirus (FV) genome. After transfection of oIFN-tau?protein was expressed in the cells. The GFP-inserted T-tropic SF2 or macrophage-tropic SF162 virus was infected to oIFN-tau transduced cell lines, and HIV-1 infectivity was measured by flow cytometry. Expression of CD4 receptor on the surface of Molt-4 cell did not change when intracellular oIFN-tau was expressed. Furthermore, oIFN-tau?gene remained over 3 months and oIFN-tau did not alter cell growth. These findings indicate that the oIFN-tau gene is able to be carried by FV vector to human cells and that the oIFN-tau DNA agent might be useful for intervention of AIDS or suppression of hepatitis virus
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