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265 HIV-1 Diversity in a Clinic at the Periphery of São Paulo Metropolitan Area
R. Rodrigues1, J. Leandro1, P. Yoshi2, N. Gaburo, Jr. 1, R. M. Custódio1, M. I. Oliveira1, R. Alcade1, and L. F. M. Brígido*1,2
1Inst. Adolfo Lutz/SP, Brazil and 2 SMS-Itap. Serra, Brazil
Introduction: HIV-1 diversity is a major issue in monitoring the epidemic and central to vaccine initiatives. We analyzed HIV genetic characteristics in a small public health service at Itapecerica, da Serra, a city in the southeast of São Paulo metropolitan area.
Methods: Blood from consecutive informed consent users was processed and cell lysades submitted to nested PCR according to UNAIDS protocol (NIH repository #2751). Sequencing was done using Big dye methodology with primers ED7, ED8, and ED33 for envelope (env) and primers for polymerase (pol) DP16, DP17, F3, and F4 after a previous nested PCR. Sequence were aligned and submitted to available sites for preliminary subtyping (www.ncbi.nih.gov).
Results: HMA env results were obtained for 17 patients (7 B, 5 F, and 5 ign); 4 of the HMA F env were sequenced, all 4 with F1 genotype. Pol regions sequence showed 2 F1 and 2 F/B pol recombination. One HMA env B was subtyped as B in pol region. The others 5 samples not analyzed by HMA were pol sequenced showing 1 B and 3 with evidence of recombination of B with F1, A, and D.
Conclusions: Albeit the small sampling, the important diversity observed thus far support the notion that the HIV epidemic, spreading in the periphery, may be distinct of that at larger centers. Larger sampling and detailed phylogenetic studies are need to confirm these preliminary findings and to delineate its impact in the evolution of HIV epidemic in the area.
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