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86 A Carbohydrate-Based Microarray System for Characterizing
AIDS-Associated Microbial Infections
D. Wang*, B. J. Trummer, S. Liu, A. Wang, and H. Zhou
Columbia Univ., New York, NY, USA
We describe the development of a carbohydrate-based microarray to extend the scope of biomedical research on carbohydrate-mediated molecular recognition and anti-infection responses. We have demonstrated that microbial polysaccharides and glycoproteins can be stably immobilized on a surface-modified glass slide without chemical conjugation. With this procedure, a large repertoire of microbial antigens can be patterned on a single micro-glass slide, reaching the capacity to include most common and conditional pathogens. Glycoconjugates of different structural characteristics are shown here to be applicable for microarray fabrication, extending the repertoires of diversity and complexity of carbohydrate microarrays. In addition, the printed carbohydrate microarrays can be air-dried and stored at room temperature for a long period of time without altering their immunological characteristics. This method is, therefore, simple, efficient, and suitable for industry scale production of carbohydrate microarrays. This technology is readily applicable for the identification of dominant antigenic determinants of HIV and for probing the repertoire of infectious agents that are associated with AIDS patients.
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