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16. Poster Session
Cross-Clade Immune Responses

Abstracts

#Title
65 Detection of HIV-1 Cross-Clade and Clade-Specific CD8 Responses from Thai and US HIV-1-Infected Individuals Using a Modified Interferon-gamma ELISPOT Assay
J. Currier*1, M. DeSouza2, P. Chanbancherd2, D. Birx1, and J. Cox1
1The US Military HIV Res. Program, Rockville, MD, USA and 2Armed Forces Inst. of Pathology, Bangkok, Thailand

66 Cell-Mediated Cross-Clade Immune Reactivity among Ethiopian Emigrants Infected with Clade C HIV-1
G. Ferrari*1, L. Earhart1, J. Currier2, L. Jagodzinski2, J. Cox2, S. Osmanov3, D. Birx2, K. Weinhold1, and S. Maayan4
1Duke Univ., Durham, NC; 2The US Military HIV Res. Prgm., Rockville, MD; 3WHO/UNAIDS, Geneva, Switzerland; and 4Hadassah Univ. Hosp., Jerusalem, Israel

67 Humoral Anti-Tat Immune Response in Individuals Infected with Different HIV-1 Clades Correlates with the Asymptomatic Stage of HIV-1 Infection and with Nonprogression to AIDS
F. Ensoli1, V. Fiorelli2, S. Buttò*2, A. Tripiciano2, A. Scoglio2, M. J. Ruiz-Alvarez2, B. Collacchi1, A. Caputo3, T. Wagner2, M. Colvin4, E. Vardas5, A. Cafaro2, F. Aiuti1, G. Rezza2, B. Ensoli2, and the Tat Multicentric Study Grp.
1Univ. of Rome “La Sapienza,” Italy; 2Inst. Superiore di Sanità, Rome, Italy; 3Univ. of Ferrara, Italy; 4Med. Res. Council, Durban, South Africa; and 5Chris Hani Baragwanath Hosp., Johannesburg, South Africa

68 Matrix-Based ELISPOT Assay for Identification of Cross-Clade HIV-1-gag T Lymphocyte Reactivity
S. M. Keating*, J. B. Jackson, R. C. Bollinger, and L. M. Carruth
Johns Hopkins Univ. Sch. of Med., Baltimore, MD, USA

69 Gag-Specific CD8+ T-Lymphocyte Immunodominant Epitopes in HIV-1-Infected Individuals from Democratic Republic of Congo
N. Lubaki*1, M. Kashamuka2, L. Musey3, L. Atibu2, B. Milangu2, N. Nzila2, L. Cheng4, H. Huiming5, S. Keating1, L. Carruth1, T. Quinn1, and R. Bollinger1
1Johns Hopkins Univ., Baltimore, MD, USA; 2Projet SIDA, Kinshasa, RDC; 3Univ. of Washington, Seattle, USA; 4CDC, Atlanta, GA, USA; and 5Emory Univ., Atlanta, GA, USA

70 HIV-1 Gag, Pol Cross-Clade-Specific Cytotoxic T Lymphocytes and gag Epitope Mapping in Asymptomatic HIV-1-Infected Thai Patients
S. Buranapraditkun*1,2, K. Ruxrungtham1,2, P. Hansasutha1,3, S. Rowland-Jones3, S. Sirivichaykul1,2, P. Warachit 4, M. Honda5, and P. Phanuphak1,2
1Chulalongkorn Univ., BKK; 2Thai Red Cross AIDS Res. Ctr., BKK; 3John Radcliffe Hosp., Oxford, UK; 4Dept. of Med. Sci., Thailand; and 5Natl. Inst. of Infectious Diseases, Tokyo, Japan

71 Both Cross-Clade Neutralization and Cross-Clade Enhancement of HIV-1 Primary Isolates Are Observed Using an Assay Based on CD4+CCR5+CXCR4+ HeLa Cells
F. Barin*, S. Brunet, D. Brand, and R. Peyre
Univ. F Rabelais, Tours, France

72 Human Anti-V3 Monoclonal Antibodies (mAbs) Selected with a V3-Fusion Protein (V3-FP) Cross-React with and Neutralize HIV-1 Primary Isolates from Various Clades
M. K. Gorny*1, C. Williams1, K. Revesz2, S. Cohen1, W. J. Honnen3, C. Krachmarov3, A. Pinter3, and S. Zolla-Pazner1,2
1New York Univ. Sch. of Med.; 2Veterans Affairs Med. Ctr.; and 3Publ. Hlth. Res. Inst., New York, NY

73 Isolation and Characterization of Multi-Clade V3-Specific Antibodies from Sera of Infected Humans That Effectively Neutralize Primary Isolates of HIV-1
C. Krachmarov1, W. Honnen1, S. Kayman1, M. Gorny2, S. Zolla-Pazner2,3, and A. Pinter*1
1Publ. Hlth. Res. Inst.; 2New York Univ. Sch. of Med.; and 3VA Med. Ctr., New York, NY, USA