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24. Poster Session
Envelope Structure/Function/Novel Immunogens

Abstracts

#Title
113 Inhibition of sCD4-Induced Conformational Changes of gp41 Ectodomain by C Peptide
L. Yi, L. Hong, and T Hattori*
Graduate Sch. of Med., Tohoku Univ., Sendai, Japan

114 Residues of GP120 Core Structure on the GP41 Interacting Face Contributing to the High Neutralization Resistance of Wild-Type Strains of HIV-1: Relevance to Vaccine Development
M. Leavitt*, E.-J. Park, and G. Quinnan
Uniformed Services Univ. of the Hlth. Sci., Bethesda, MD, USA

115 Membrane Permeabilization by the HIV-1 gp41 Cytoplasmic LLP Domains Differs by Subtype
W. Dowling*1, M. Robb1, D. Birx1, and F. McCutchan2
1Walter Reed Army Inst. of Res., Washington, DC, USA and 2Henry M. Jackson Fndn., Rockville, MD, USA

116 In Vivo Alteration of the Highly Conserved 370 Residue of Human Immunodeficiency Virus Type 1 gp120 Determines Neutralization Sensitivity against CD4-Bnding Site and gp41-Directed Agents
T. Beaumont*1, A. van Nuenen1, S. Broersen1, D. Schols2, and H. Schuitemaker1
1CLB Sanquin and Univ. of Amsterdam, The Netherlands and 2Rega Inst. for Med. Res., Leuven, Belgium

117 A Chimeric Protein of SIV Envelope Glycoprotein Gp-160e and E. coli Aspartate Transcarbamoylase
B. Chen*1, J.J. Skehel3, E.L. Reinherz4, S.C. Harrison1,2, and D.C. Wiley1,2
1The Children's Hosp./Harvard Univ., Boston, MA, USA; 2 Howard Hughes Med. Inst., Boston, MA, USA; 3Natl. Inst. for Med. Res., London, UK; and 4Dana Farber Cancer Inst., Boston, MA, USA

118 Structural Flexibility and Functional Valency of CD4-IgG2 (PRO 542) and CD4- gamma2: Potential for Crosslinking HIV-1 Envelope Spikes
K. H. Roux*1, Z. Ping1, and W. C. Olson2
1Florida State Univ., Tallahassee, USA and 2Progenics Pharma., Inc., Tarrytown, NY, USA

119 Selective Mutations of the gp120 Glycosylations Sites on an Acute HIV-1 Infection Primary Isolate env Sequence According to their Clustering on the 3D Model
F. Bedin, Y. Ataman-Önal, F. Reynard, P.-Y. Durand, M. Girard, and B. Verrier*
CNRS-BioMerieux, CERVI, Lyon, France

120 Structural Features of HIV Envelope Defined by Antibody Escape Mutant Analysis
S. D’Costa*1, K. S. Slobod1, R. G. Webster1, S. White1, and J. Hurwitz1,2
1St Jude Children’s Res. Hosp., Memphis, TN, USA and 2Univ. of Tennessee, Memphis, USA

121 Effects of Glycosylation Variation on Envelope-Specific Antibody Responses
K. S. Cole*1, J. D. Steckbeck 1, J. L. Rowles1, R. C. Desrosiers2, and R. C. Montelaro1
1Univ. of Pittsburgh Sch. of Med., PA, USA and 2New England Regional Primate Res. Ctr., Southborough, MA, USA

122 Chemically Inactivated HIV/SIV Virions with Functional Envelope Glycoproteins: In Vitro Applications
J. L. Rossio*1, M. Piatak, Jr.1, I. Frank2, M. Larsson2, N. Romani3, L. Henderson1, L. Arthur1, N. Bhardwaj2, M. Pope2, and J. D. Lifson1
1AVP, SAIC Frederick, NCI at Frederick, MD, USA; 2Rockefeller Univ., New York, NY, USA; and 3Univ. of Innsbruck, Austria

123 Selected Mutations to Remove the Potential N-Linked Glycosylation Sites at the V1-Loop of HIV-1 Envelope Glycoprotein Were Able to Induce Neutralizing Antibody Responses against the Primary HIV Isolate
S. Wang, I. Mboudjeka, S. Shen, T. Wu-Chou, T. Giehl, J. Brinker, and S. Lu*
Univ. of Massachusetts Med. Sch., Worcester, USA

124 Production, Characterization, and Oligomeric Stabilization of Recombinant Disulfide Linked (SOS) HIV-1 Envelope Glycoproteins
N. Schülke*1, M. Vesanen2, R. Sanders2, A. Villa1, D. Anselma1, J. Binley3, P. Maddon1, J. Moore2, and W. Olson1
1Progenics Pharmaceuticals Inc., Tarrytown, NY, USA; 2Weill Med. Coll., Cornell Univ., New York, NY, USA; and 3The Scripps Res. Inst., La Jolla, CA, USA

125 Identification of a Fusion Intermediate Conformation of Soluble HIV Envelope gp41
M. Alam*1, H.-X. Liao1, C. Paleos1, S. Stauffer1, R. Scearce1, P. Ear2, and B. Haynes1
1Duke Med. Ctr., Durham, NC, USA and 2 NIAID, NIH, Bethesda, MD, USA

126 Combination of Monophosphoryl Lipid A and GM-CSF Adjuvant with an HIV Envelope Immunogen Coupled to a2-Macroglobulin Dramatically Increases HIV Envelope Subunit Immunogenicity
H.-X. Liao*, G. J. Ciancioloý, H. F. Staats, R. M. Scearce, D. M. Lapple, S. Stauffer, J. R. Thomasch, S. V. Pizzoý, D. C. Montefiori, and B. F. Haynes
Duke Univ. Med. Ctr., Durham, NC, USA

127 Mapping of the Epitopes on HIV-1 gp120 Recognized by Neutralizing Human Monoclonal Antibodies by Alanine-Scanning Mutagenesis
R. Pantophlet*, E. Ollmann Saphire, I. A. Wilson, P. W. H. I. Parren, and D. R. Burton
The Scripps Res. Inst., La Jolla, CA, USA

128 Immunogenicity of Conjugated HIV Gp120 and Env 2-3 in Mice
L. C. Paoletti*1 and R. C. Kennedy2
1Brigham & Women’s Hosp., Harvard Med. Sch, Boston, MA, USA and 2Texas Tech Univ. Hlth. Sci. Ctr., Lubbock, TX, USA

129 Effect of Variable Loop Deletion on Envelope Glycoprotein-Specific Cellular Responses and Protection against Challenge with Recombinant Vaccinia Virus Expressing gp160 of Primary HIV-1 Isolates
J. Gzyl*, I. Kiszka, D. Kmieciak, E. Bolesta, T. Naito, and D. Kozbor
Temple Univ., Philadelphia, PA, USA