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56. Slide Session
Innovative Approaches to HIV Vaccine Research: Highlighted NIAID Innovation Grants
Moderators: James Bradac, NIAID, NIH Bethesda, MD, USA and Jamie Scott, Simon Fraser Univ., Burnaby, BC, Canada

Abstracts

#Title
289 Dendritic Cell Amplification of Cryptic HIV-1-Specific T-Cell Responses in Exposed, Seronegative Individuals
B. L. Shacklett*1,5, R. Means2, M. Larsson3, D. Wilkens1, T. Beadle1, M. Merritt1, N. Bhardwaj3, J. Skurnick4, D. Louria4, P. Palumbo4, and D. F. Nixon1-5
1Aaron Diamond AIDS Res. Ctr., New York, NY, USA; 2New England Primate Res. Ctr., Southborough, MA, USA; 3Rockefeller Univ., New York, NY, USA; 4New Jersey Med. Sch., Newark, USA; and 5Gladstone Inst. of Virology and Immunology, San Francisco, CA, USA

290 Identification and Optimization of HIV-Specific CTL Antigens for Vaccine Design
C. Boggiano*1, C. Pinilla1, B. D. Walker2, and S. E. Blondelle1
1Torrey Pines Inst. for Molecular Studies, San Diego, CA, USA and 2Partners AIDS Res. Ctr., Harvard Med. Sch., Charlestown, MA, USA

291 Construction and Biological Function of the gp120 Bridging Sheet
C. H. Chen* and C. B. Zhu
Meharry Med. Coll., Nashville, TN, USA

292 Humoral and Cellular Immune Responses Induced by HIV Tat Protein Fails to Confer Protection against SHIV89.6p Infection
P. Silvera*1, J. Greenhouse1, J. Yalley-Ogunro1, M. W. Richardson2, J. Mirchandani2, E. G. Regulier2, C. Capini2, K. Khalili2, J.-F. Zagury3, M. Lewis1, and J. Rappaport2
1Southern Res. Inst., Frederick, MD, USA; 2Temple Univ., Philadelphia, PA, USA; and 3Univ. Pierre et Marie Curie, Paris, France

293 Protective Immunity in Rhesus Monkeys Immunized with Replication-Defective HIV-1 Vaccine
F. Tung*1, K. Cole2, L. Tung1, H. McClure3, and R. Montelaro2
1GeneCure LLC, Atlanta GA, USA; 2Univ. of Pittsburgh, PA, USA; and 3Emory Univ., Atlanta GA, USA

294 Stabilizing Cleaved Forms of Env in Virus-Like Particles
J. M. Binley*1, A. Master2, L. Schiffner2, D. Schiller2, M. Paluch2, B. Travis3, S.-L. Hu3, W. Olson4, and J. P. Moore2
1The Scripps Res. Inst., La Jolla, CA, USA; 2Weill Med. Coll., Cornell Univ., New York, NY, USA; 3Univ. of Washington, Seattle, USA; and 4Progenics Pharmaceuticals, Tarrytown, NY, USA

295 B2.1, a Peptide That Specifically Binds the Broadly HIV-1-Neutralizing, Human Antibody, b12
A. Menendez1, M. Zwick 1, L. Bonnycastle 1, M. Irving 1, M. Montero 1, C. Barbas 2, P. Parren 2, D. Burton 2, and J. Scott* 1
1Simon Fraser Univ., Burnaby, BC, Canada and 2The Scripps Res. Inst., La Jolla, CA, USA

296 A Novel Vaccine to HIV-1 Gag Based on a Replication-Defective Adenovirus Recombinant of the Chimpanzee Serotype 68
J. Fitzgerald1, J. Wilson2, and H. Ertl*1
1The Wistar Inst., Philadelphia, PA, USA and 2Univ. of Pennsylvania, Philadelphia, USA

297 HIV-HPV Chimeric Virus-Like Particles Induce HIV-Specific Mucosal and Systemic CTL Responses
J. Liu, W. Shi, W. Li, Y. Huang, M. Velders, W. M. Kast, D. Quinn, M. Mueller, and L. Qiao*
Loyola Univ. Chicago, Maywood, IL, USA

298 Vaccination against SIV with tat and rev
K. J. Stittelaar1, R. A. Gruters1,2, M. Schutten1, C. A. van Baalen1, G. van Amerongen1, M. Cranage3, P. Liljeström4, G. Sutter5, and A. D. M. E. Osterhaus*1
1Erasmus Med. Ctr., Rotterdam, The Netherlands; 2UMR2142, CNRS/BioMérieux, ENS Lyon, France; 3CAMR, Porton Down, Salisbury, Wilts, UK; 4Microbiology and Tumorbiology Ctr., Karolinska Inst., Stockholm, Sweden; and 5GSF-Inst. of Molecular Virology, Neuherberg, Germany

299 Immunogenicity in Neonatal Mice of a Hyperattenuated Listeria Vaccine Directed against HIV
M. Rayevskaya, N. Kushnir, and F. R. Frankel*
Univ. of Pennsylvania Sch. of Med., Philadelphia, USA

300 Highly Attenuated Shigella Is Superior to Attenuated Salmonella for Mucosal DNA Vaccination against HIV
W. Vecino*1, P. Morin1, W. Jacobs, Jr.1,2, and G. Fennelly1
1Albert Einstein Coll. of Med. and 2Howard Hughes Med. Inst., Bronx, NY, USA